ADDI's Editorial Take

What is it and what does it include? 

Human induced pluripotent stem cell (iPSC) lines are a powerful tool for studying development and disease, but the considerable phenotypic variation between lines makes it challenging to replicate key findings and integrate data across research groups. In this study, investigators identified KOLF2.1J as a candidate reference cell line based on single-cell analysis of its gene expression in the pluripotent state, whole genome sequencing, genomic stability after highly efficient CRISPR-mediated gene editing, integrity of the p53 pathway, and the efficiency with which it differentiated into multiple target cell populations. Since it is deeply characterized and can be readily acquired, KOLF2.1J is an attractive reference cell line for groups working with iPSCs. This dataset includes the whole genome and single cell RNA sequencing files from our analyses. Temporal proteomic profiling of iPSC-derived neurons from the KOLF2.1J parental line over a course of 28-day differentiation (n = 6), including 7 time points (day0 [d0], d3, d7, d10, d14, d21, d28) are also available. 

How can I use this dataset to advance my research? 

This dataset is ideal if: 

  • you’re seeking to work with a trusted iPSC line to further the larger aim of improving reproducibility and collaboration in the field.